MDScan™ Microarray Technology

MDScan™ (GenomicTree) technology for the systematic and comprehensive genome-wide discovery of epigenetically silenced genes uses affinity-based methyl DNA enrichment, a bisulfite-free method, for selective enrichment of methylated DNA. The selectively isolated methyl DNA can be used for microarray analysis. This technology does not depend on bisulfite modification, restriction endonucleases or specific antibodies and will lead to the discovery of novel methylation biomarkers for early detection of cancer, determination of tumor stage, risk of recurrence, and prediction of response to drug therapy.

Mucins as Epigenetic Biomarkers in Epithelial Cancers

Genes encoding mucins have been shown to be regulated by DNA methylation and histone modifications in epithelial cancer cells. These genes encode either secreted glycoproteins necessary for epithelial homeostasis or membrane-bound glycoproteins that participate in tumor progression. The important biological functions played by these large molecules in pathophysiology of the epithelia make them key genes to target for new therapeutic strategies and new diagnostic and/or prognostic tools in cancer. Mucin genes may be regulated by miRNAs and also regulate miRNA activity. Epigenetic regulation of mucin genes has a great potential to provide new diagnostic as well as prognostic biomarkers, help tumor classification and form the basis of new therapeutic targets for the clinician and the pathologist (Van Seuningen and Vincent 2009).

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