Application of BS for Vegetative Propagation of Perennial Plants

Another most interesting quality of BS was its ability to actively induce the root formation of woody plants cuttings. The cuttings of woody plants growing in the desert, semi-desert and steppe and arid regions of our republic were specially taken for these experiments.

For vegetative propagation of plants the biostimulants developed on the basis of auxin and its analogues are used today. The main disadvantage of these stimulants is that they cause the formation of only lateral roots and inhibiting the development of main root. Therefore, these stimulants are unsuitable for propagation of plants, having a core root system. But the majority of steppe woody plants in Kazakhstan have a core root system. They must go deep to earth in order to reach the horizon containing the moisture. Therefore, auxin biostimulants for vegetative propagation of such plants are not suitable. Our experiments have shown that BS has the following advantages over all previously known stimulants for vegetative propagation:

BS does not have the polarity property. Auxins are resulting the root growth, but suppress the growth of leaves and shoots. Cytokines are affected on shoot growth, but do not affect the growth of roots. Our superbiostimulant is equally stimulating the growth of roots, and leaves and new stems. So the use of BS allows to get a fully formed wood planting material much earlier.

Another advantage of SBS is that it is absolutely non-toxic, and is not a chemically synthetic compound. While auxins are toxic and carcinogenic. Auxins especially 2,4-dinitrophenoxy acetic acid (2,4-D) are toxic and endogenous

Thus, BS has undeniable advantages over the auxin biostimulators for successful vegetative reproduction of perennial plants. Vegetative multiplication of perennial plants has a number of clear advantages over the seed propagation method. Thus, the plants grown by vegetative propagation grow faster and bear fruit 2-3 у faster than plants obtained by seed. In addition, these plants are absolutely adapted to environmental and climatic conditions, than the plants obtained at seed propagation

And finally, the vegetative propagation allows to obtain the exact clones of plants with outstanding economic characteristics. In order to test the effectiveness of BS usage for vegetative propagation of woody plants are capable to grow in desert, semi- desert and steppe conditions. For the experiment, the cuttings of the following plants were taken: Tamarix ramosissima, Sea- buckthorn (Hippophae rhamnoides L). Cuttings of these plants were soaked in a solution BS of 50 ng/ml during the day. Then cuttings were transferred to water and grown for a month. The water changed per one week. The results of these experiments are presented in Figs. 6.11 and 6.12.

When analyzing the obtained results, the attention should be paid to the following. BS has a stimulating effect not only on root formation, but also on the growth and development of aboveground part of the cuttings. This suggests that the usage of BS for vegetative propagation allows to get a fully formulated planting material in a short time. These results suggest a high efficiency of BS for vegetative propagation of woody plants are able to grow in dry conditions of Kazakhstan. It should be noted that for vegetative propagation of a huge amount of planting material, there are needed only grams of the BS preparation.

Rooting of Tamarix ramosissima using BS Tamarix ramosissima)

Figure 6.11 Rooting of Tamarix ramosissima using BS Tamarix ramosissima): (1) experience and (2) control.

Rooting of Hippophae rhamnoides using BS (Hippophae rhamnoides L)

Figure 6.12 Rooting of Hippophae rhamnoides using BS (Hippophae rhamnoides L): (1) experience and (2) control.

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