SECTION I: Chemistry of Plant Volatiles

Practical Approaches to Plant Volatile Collection and Analysis

Dorothea Tholl, Alexander Weinhold, and Ursula S. R. Rose

CONTENTS

  • 1.1 Introduction..............................................................................................................................3
  • 1.2 Plant Volatile Sampling Techniques.........................................................................................4
  • 1.2.1 Static Headspace or In Situ Sampling Techniques.......................................................5
  • 1.2.1.1 Solid Phase Microextraction..........................................................................5
  • 1.2.1.2 PDMS-Based Silicone Tubing.......................................................................6
  • 1.2.1.3 Use of Stir-Bar Sorptive Extraction (SBSE) in Headspace Sampling...........7
  • 1.2.2 Dynamic Headspace Sampling Techniques.................................................................7
  • 1.2.2.1 Closed-Loop Stripping...................................................................................7
  • 1.2.2.2 Pull Systems...................................................................................................8
  • 1.2.2.3 Push-Pull Systems.........................................................................................9
  • 1.3 Adsorption and Desorption of Volatiles.................................................................................10
  • 1.4 Gas Chromatographic Separation of Volatiles.......................................................................11
  • 1.5 Volatile Detection and Identification......................................................................................12
  • 1.5.1 Enantioselective GC, Multidimensional GC..............................................................13
  • 1.6 Volatile Analysis Techniques with High Time Resolution.....................................................14
  • 1.6.1 Transportable GC.......................................................................................................14
  • 1.6.2 Proton-Transfer Reaction Mass Spectrometry (PTR-MS)..........................................14
  • 1.6.3 Automated Systems for Volatilome Analysis.............................................................16
  • 1.6.4 Sensors for VOC Profiling in the Field.......................................................................17
  • 1.7 Special Considerations for Belowground Volatile Collection................................................18
  • 1.8 Specific Considerations in Aquatic Systems..........................................................................19
  • 1.9 Conclusions.............................................................................................................................21

References........................................................................................................................................21
 
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