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Home arrow Health arrow The Impact of Food Bioactives on Health

A short course of lectures
«The Impact of Food Bioactives on Health»

Assess ViabilityHistory and Special Features of the ModelQuality in Relation to Other Models with the Same ApplicabilityFeatures and MechanismsAdvanced Gastric Compartment (TIM-agc)Stability and Reproducibility of the SystemDendritic CellsIn Vivo Digestion of Infant Formula on PigletsSample PreparationMedium QualityGeneral ProtocolThe TNO Gastro-Intestinal Model (TIM)Description of the ModelOriginSolubility/DialyzabilityExperimental Read OutAssess ViabilityPeripheral Blood Mononuclear CellsUse of Digested Food SamplesStability, Consistency and Reproducibility of the SystemRelevance to Human In Vivo SituationTransport StudiesDifferentiation of THP-1 and U937 Monocytes into MacrophagesOriginTemperature ControlAvailability of the SystemTroubleshooting Guide for Transport Experiments Across Caco-2 MonolayersGastric SecretionsCell Viability Testing-D Culture SystemsDifferentiation of THP-1 and U937 Monocytes into Dendritic CellsCo-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events)Model DescriptionRegulation of GLUTag Cell Secretory Activity by Other SignalsEverted SacGastrointestinal OrganoidsControls: Positive and NegativeCaco-2/THP-1 (Soluble Factors)Serum AdditionThe Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)Controls to Test Stability and Performance of the ModelGeneral Protocol for DGM ExperimentsBiochemistryIsolating Primary DCs from BloodApproaches to Static Digestion ModelsExperimental Protocol for Test CompoundsSpecial FeaturesThe Porcine Ex Vivo Intestinal Segment ModelUses of the DGMRead-Out of the SystemDC Cell LinesPhysical ForcesExperimental ReadoutsSTC-1 CellsRead-Out of the System and How This Information Can Be UsedGeneral Protocol for HT29-MTX CellsRelevance to Human In Vivo SituationStudy of Inflammatory ResponsesDigestion ConditionsExperimental ReadoutGeneral ProtocolsT Lymphocytes or T-CellsReadout of the Ussing Chamber SystemGeneral Protocol of Culturing THP-1 CellsHistory of the ModelSamplesControls to Test Stability and Performance of the ModelMaintenance of the GLUTag Cell LineStability, Consistency and ReproducibilityCaco-2/B CellsHT29 Cell LineFreezing CellsQuality in Relation to Other Models with the Same ApplicabilityRead Out of the SystemAdvantages, Disadvantages and Limitations of the SystemVerification of Monolayer Integrity by Lucifer Yellow FluxRelevance to the Human Situation (L-Cell In Vivo)Assess ViabilityMicroorganisms Survival, Adhesion or InvasionProtein QualityOrigins and Design of the DIDGI® SystemMonitoring ViabilityAdvantages, Disadvantages and Limitations of the SystemRead-Out of the System and How This Information Can Be UsedDescription of TIM-2Asses ViabilityStability and Reproducibility of the SystemGeneral ProtocolAvailability of the SystemExperimental ReadoutQuality in Relation to Other Models with the Same ApplicabilityMonitoring ViabilityExperimental Protocol for Test CompoundsReadout of the Porcine Ex Vivo Intestinal Segment ModelSpecial Features of the ModelStability, Consistency and ReproducibilityRelevance to the Human In Vivo SituationHuman Peripheral Blood Mononuclear CellsRelevance to Human In Vivo SituationUssing ChamberPrediction of Glycemic ResponseThe Ussing ChamberStability/Consistency/Reproducibility of the SystemGastric ProcessingSample AnalysisStability/Consistency and ReproducibilityOriginStatic MethodsGeneral ProtocolAdvantages, Disadvantages and LimitationsStatic Models for Lipid HydrolysisStability/Consistency/ReproducibilityOriginCritical NotesExperimental Read OutDendritic CellsCD34+-Derived DCsExperimental Read outMeasurement of Transepithelial Electrical Resistance (TEER)General MaintenanceRole of MicrobiotaAdvantages and LimitationsOrigins and Design of the DGMUses of the HGSDescription of the VTT One Compartment Fermentation ModelCell Maintenance ProtocolQuality in Relation to Other Models with the Same ApplicabilityAssess ViabilitySmall Intestinal Organoid PassageThe Small Intestinal PhaseSpecial Features/Morphology/ReceptorsAdvantages, Disadvantages and Limitations of the SystemGeneral Protocols for Caco-2 CellsAdvantages, Disadvantages and LimitationsThe Cell Culture HoodGeneral Advantages and Limitations of Intestinal Organoid SystemsControls to Test Stability and Performance of the ModelCell Culture Terminology (Fig. 1)Other Models with the Same ApplicabilitySpecial Features of the ModelCulture ConditionsGeneral ProtocolDynamic Fermentation ModelsIn Vitro Studies on Food Bioactives Using DCs (Table 17.3)Food-Based ResearchTIM-1PBMC-Derived T CellsStatic Models for Protein HydrolysisGeneral ProtocolDynamic Gastric Model (DGM)The Thawing and Recovery of CellsFunctionality StudiesCo-stimulationCaco-2 Cell LineGeneral Advantages and Disadvantages of the ModelApplicationsAssess ViabilityRelevance to Human In Vivo SituationConcept of TIMCell Maintenance ProtocolBasic ProtocolAssess ViabilityThe Computer-Controlled Multicompartmental Dynamic Model of the Gastrointestinal System SIMGIHistory of the ModelGeneral ProtocolCells Present in the IntestinePharmaceutical-Based ResearchBasic ProtocolOrigin and Some Features of THP-1 and U937 CellsCritical NotesGeneral ProtocolStability, Consistency and ReproducibilityMonocyte-Derived DCsFeatures and MechanismsIntestinal Crypt Organoids as Experimental ModelsExperimental Protocol for Test CompoundsExperimental ReadoutAssess ViabilityAdvantages, Disadvantages and Limitations of the SystemPorcine Ex Vivo Intestinal Segment ModelGastric MotilityCaco-2/Human Monocyte Derived DCs (Soluble Factors)Co-culture Caco-2/Immune CellsOriginAnalysis of HGS Biomechanical RelevanceLipidsStability, Consistency and ReproducibilityCo-cultivation of Caco-2 and HT-29MTXTiny TIMGastric EmptyingGeneral Cell Culture ProtocolsMonitoring ViabilityThe Oral PhasePassaging of Cells in Suspension CultureTrypsinisation and Subculturing of CellsCell Maintenance ProtocolComparison In Vitro/In Vivo DataAdvantages and DisadvantagesValidation of the SystemOrigins of the HGSRelevance to Human In Vivo SituationAdvantages, Disadvantages and LimitationsValidation of DIDGI® for the Digestion of Infant FormulaCytokine ProductionAdvantages, Disadvantages and LimitationsThe IPEC-J2 Cell LineOriginT Cell Isolation ProtocolsMonocytes and MacrophagesIsolated and Perfused Intestinal SegmentsPerformance of Secretion and Expression AssaysCo-culture Caco-2 and Dendritic CellsDC SubsetsOther DC ReadoutsUssing ChamberGeneral ProtocolInfoGest Consensus MethodPreparation of a Food BolusThe DIDGI® SystemSmall Intestinal Crypt IsolationGeneral ProtocolSmall Intestinal Organoid CultureDescription of SHIME®Stability, Consistency and ReproducibilitySamplingHistory of the ModelFeatures and MechanismsThe Use of TIM to Study the Bio-accessibility of NutrientsStudy of Proliferative/Cytotoxic ActivityMurine GLUTag CellsExperimental Readout of the SystemPracticalitiesCo-culture Caco-2 and B-cells (Raji)Features and MechanismsCalculating Transepithelial ResistanceOrigin, Features and MechanismsGeneral ProtocolNCI-H716 CellsOrigin, Features and MechanismsOriginIncubation with Food Components and Digested Food SamplesProtocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® SystemRelevance to the Human L-Cell In VivoOther Static ModelsThe TNO In Vitro Model of the Colon (TIM-2)Features and MechanismsRelevance to Human In Vivo SituationIntestinal Rings and Intestinal SegmentsThe Gastric PhasepH and CO2Features and MechanismsIndirect Positive Isolation of Human CD4+ TGeneral Advantages, Disadvantages and Limitations of the Ussing Chamber SystemExperimental ReadoutGeneral ProtocolHost-Gut Microbiota InteractionFeatures and MechanismsRegulation of GLUTag Cell Secretory Activity by NutrientsRole of ACW Activity on Food DigestionApplications of T Cell CulturesGastric CompartmentHuman Gastric Simulator (Riddet Model)OriginContamination of Cell CulturesStability and Reproducibility of the SystemRelevance to Human In Vivo SituationFeatures and MechanismsOperating ProtocolGeneral ProtocolRelevance to Human In Vivo SituationRelevance to Human In Vivo SituationGeneral ProtocolGeneral ProtocolApplications: Advantages and DisadvantagesCaco-2/PBMCs (Soluble Factors)Inoculation of Gut Fermentation ModelsGeneral ProtocolRole of Food Material PropertiesRelevance to Human In Vivo SituationControls to Test Viability and Performance of the ModelProtocol for Polarizing Caco-2 Cells in Tissue Culture InsertsOne Compartment Fermentation ModelRelevance to Human In Vivo SituationRead-Out of the SystemExperimental Readout
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