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The Impact of Food Bioactives on Health

A short course of lectures
«The Impact of Food Bioactives on Health»

Controls: Positive and Negative Small Intestinal Organoid Passage Use of Digested Food Samples Features and Mechanisms Cell Maintenance Protocol Gastric Compartment Model Description Intestinal Crypt Organoids as Experimental Models Cells Present in the Intestine Protein Quality Basic Protocol Experimental Protocol for Test Compounds The TNO Gastro-Intestinal Model (TIM)Approaches to Static Digestion Models Human Gastric Simulator (Riddet Model)In Vitro Studies on Food Bioactives Using DCs (Table 17.3)Origin Stability and Reproducibility of the System The Oral Phase Other Static Models Digestion Conditions Gastrointestinal Organoids Experimental Readout Protocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® System Availability of the System Study of Inflammatory Responses General Protocol pH and CO2 Origins and Design of the DGM Relevance to the Human Situation (L-Cell In Vivo)Everted Sac Role of Food Material Properties Cell Maintenance Protocol Origins of the HGS Caco-2/B Cells Role of ACW Activity on Food Digestion Applications General Protocol DC Cell Lines Origins and Design of the DIDGI® System Caco-2 Cell Line Experimental Read out Sample Analysis General Protocol Origin, Features and Mechanisms Readout of the Porcine Ex Vivo Intestinal Segment Model Critical Notes Read-Out of the System and How This Information Can Be Used Relevance to the Human L-Cell In Vivo Advantages and Disadvantages Lipids General Protocol of Culturing THP-1 Cells The Thawing and Recovery of Cells Static Models for Lipid Hydrolysis Co-culture Caco-2 and B-cells (Raji)Peripheral Blood Mononuclear Cells HT29 Cell Line Static Models for Protein Hydrolysis Calculating Transepithelial Resistance History and Special Features of the Model Features and Mechanisms The Porcine Ex Vivo Intestinal Segment Model Assess Viability Relevance to Human In Vivo Situation Role of Microbiota CD34+-Derived DCs Differentiation of THP-1 and U937 Monocytes into Dendritic Cells History of the Model The IPEC-J2 Cell Line Uses of the DGM Origin, Features and Mechanisms Stability/Consistency and Reproducibility Assess Viability Comparison In Vitro/In Vivo Data TIM-1 Advanced Gastric Compartment (TIM-agc)Assess Viability Solubility/Dialyzability Assess Viability Transport Studies Advantages, Disadvantages and Limitations of the System Experimental Readout Small Intestinal Organoid Culture Gastric Secretions Regulation of GLUTag Cell Secretory Activity by Nutrients General Protocol Features and Mechanisms Experimental Read Out General Protocol Gastric Emptying Read Out of the System Origin Host-Gut Microbiota Interaction Advantages, Disadvantages and Limitations of the System History of the Model The Small Intestinal Phase Stability, Consistency and Reproducibility Static Methods Dendritic Cells Monitoring Viability Features and Mechanisms Isolating Primary DCs from Blood Troubleshooting Guide for Transport Experiments Across Caco-2 Monolayers Stability, Consistency and Reproducibility of the System Indirect Positive Isolation of Human CD4+ T The Use of TIM to Study the Bio-accessibility of Nutrients Operating Protocol Experimental Readout Features and Mechanisms Critical Notes InfoGest Consensus Method -D Culture Systems Caco-2/Human Monocyte Derived DCs (Soluble Factors)Advantages, Disadvantages and Limitations of the System Stability, Consistency and Reproducibility Verification of Monolayer Integrity by Lucifer Yellow Flux Caco-2/PBMCs (Soluble Factors)Relevance to Human In Vivo Situation Origin and Some Features of THP-1 and U937 Cells General Protocol Cytokine Production Description of the VTT One Compartment Fermentation Model General Protocols Advantages and Limitations Physical Forces Origin Description of TIM-2 Medium Quality Trypsinisation and Subculturing of Cells Culture Conditions Advantages, Disadvantages and Limitations Human Peripheral Blood Mononuclear Cells Relevance to Human In Vivo Situation The TNO In Vitro Model of the Colon (TIM-2)General Protocol Dynamic Gastric Model (DGM)Advantages, Disadvantages and Limitations Sample Preparation Asses Viability The Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)One Compartment Fermentation Model Description of the Model Origin Preparation of a Food Bolus Relevance to Human In Vivo Situation Controls to Test Stability and Performance of the Model General Protocol Quality in Relation to Other Models with the Same Applicability Cell Viability Testing Applications: Advantages and Disadvantages Features and Mechanisms Relevance to Human In Vivo Situation The Ussing Chamber Description of SHIME®NCI-H716 Cells Relevance to Human In Vivo Situation Freezing Cells Controls to Test Viability and Performance of the Model Relevance to Human In Vivo Situation Stability and Reproducibility of the System Concept of TIM History of the Model Assess Viability Special Features of the Model Controls to Test Stability and Performance of the Model Contamination of Cell Cultures Controls to Test Stability and Performance of the Model PBMC-Derived T Cells Biochemistry Small Intestinal Crypt Isolation General Protocols for Caco-2 Cells Validation of the System Experimental Readout T Lymphocytes or T-Cells Inoculation of Gut Fermentation Models Prediction of Glycemic Response Study of Proliferative/Cytotoxic Activity Monocytes and Macrophages Functionality Studies Co-culture Caco-2 and Dendritic Cells Gastric Processing Readout of the Ussing Chamber System Relevance to Human In Vivo Situation Stability and Reproducibility of the System Advantages, Disadvantages and Limitations Origin Read-Out of the System and How This Information Can Be Used DC Subsets Read-Out of the System Passaging of Cells in Suspension Culture Caco-2/THP-1 (Soluble Factors)General Protocol Features and Mechanisms The DIDGI® System Stability/Consistency/Reproducibility Origin General Advantages, Disadvantages and Limitations of the Ussing Chamber System Cell Maintenance Protocol Basic Protocol Samples Co-culture Caco-2/Immune Cells Monocyte-Derived DCs Performance of Secretion and Expression Assays Experimental Readout Quality in Relation to Other Models with the Same Applicability Experimental Protocol for Test Compounds Dynamic Fermentation Models General Protocol Protocol for Polarizing Caco-2 Cells in Tissue Culture Inserts Dendritic Cells Special Features General Protocol for DGM Experiments Temperature Control Co-stimulation Relevance to Human In Vivo Situation Co-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events)General Cell Culture Protocols Analysis of HGS Biomechanical Relevance General Protocol Practicalities Maintenance of the GLUTag Cell Line General Protocol Applications of T Cell Cultures General Advantages and Disadvantages of the Model Intestinal Rings and Intestinal Segments Porcine Ex Vivo Intestinal Segment Model Pharmaceutical-Based Research Monitoring Viability Gastric Motility Monitoring Viability Incubation with Food Components and Digested Food Samples Measurement of Transepithelial Electrical Resistance (TEER)Murine GLUTag Cells STC-1 Cells Isolated and Perfused Intestinal Segments Tiny TIM Advantages, Disadvantages and Limitations of the System Special Features of the Model Experimental Readouts Relevance to Human In Vivo Situation Differentiation of THP-1 and U937 Monocytes into Macrophages Co-cultivation of Caco-2 and HT-29MTX Assess Viability Experimental Read Out Relevance to the Human In Vivo Situation Stability, Consistency and Reproducibility Quality in Relation to Other Models with the Same Applicability The Cell Culture Hood Cell Culture Terminology (Fig. 1)Serum Addition Special Features/Morphology/Receptors Sampling Assess Viability Other DC Readouts Origin General Protocol for HT29-MTX Cells Experimental Readout of the System T Cell Isolation Protocols Quality in Relation to Other Models with the Same Applicability General Advantages and Limitations of Intestinal Organoid Systems General Protocol Relevance to Human In Vivo Situation Stability/Consistency/Reproducibility of the System Regulation of GLUTag Cell Secretory Activity by Other Signals The Computer-Controlled Multicompartmental Dynamic Model of the Gastrointestinal System SIMGI The Gastric Phase Other Models with the Same Applicability Stability, Consistency and Reproducibility Read-Out of the System Uses of the HGS Experimental Protocol for Test Compounds Availability of the System General Protocol Validation of DIDGI® for the Digestion of Infant Formula Stability, Consistency and Reproducibility In Vivo Digestion of Infant Formula on Piglets Food-Based Research Features and Mechanisms General Protocol Advantages, Disadvantages and Limitations Origin Ussing Chamber General Maintenance Microorganisms Survival, Adhesion or Invasion Ussing Chamber

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