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Home arrow Health arrow The Impact of Food Bioactives on Health

A short course of lectures
«The Impact of Food Bioactives on Health»

Controls: Positive and NegativeSmall Intestinal Organoid PassageUse of Digested Food SamplesFeatures and MechanismsCell Maintenance ProtocolGastric CompartmentModel DescriptionIntestinal Crypt Organoids as Experimental ModelsCells Present in the IntestineProtein QualityBasic ProtocolExperimental Protocol for Test CompoundsThe TNO Gastro-Intestinal Model (TIM)Approaches to Static Digestion ModelsHuman Gastric Simulator (Riddet Model)In Vitro Studies on Food Bioactives Using DCs (Table 17.3)OriginStability and Reproducibility of the SystemThe Oral PhaseOther Static ModelsDigestion ConditionsGastrointestinal OrganoidsExperimental ReadoutProtocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® SystemAvailability of the SystemStudy of Inflammatory ResponsesGeneral ProtocolpH and CO2Origins and Design of the DGMRelevance to the Human Situation (L-Cell In Vivo)Everted SacRole of Food Material PropertiesCell Maintenance ProtocolOrigins of the HGSCaco-2/B CellsRole of ACW Activity on Food DigestionApplicationsGeneral ProtocolDC Cell LinesOrigins and Design of the DIDGI® SystemCaco-2 Cell LineExperimental Read outSample AnalysisGeneral ProtocolOrigin, Features and MechanismsReadout of the Porcine Ex Vivo Intestinal Segment ModelCritical NotesRead-Out of the System and How This Information Can Be UsedRelevance to the Human L-Cell In VivoAdvantages and DisadvantagesLipidsGeneral Protocol of Culturing THP-1 CellsThe Thawing and Recovery of CellsStatic Models for Lipid HydrolysisCo-culture Caco-2 and B-cells (Raji)Peripheral Blood Mononuclear CellsHT29 Cell LineStatic Models for Protein HydrolysisCalculating Transepithelial ResistanceHistory and Special Features of the ModelFeatures and MechanismsThe Porcine Ex Vivo Intestinal Segment ModelAssess ViabilityRelevance to Human In Vivo SituationRole of MicrobiotaCD34+-Derived DCsDifferentiation of THP-1 and U937 Monocytes into Dendritic CellsHistory of the ModelThe IPEC-J2 Cell LineUses of the DGMOrigin, Features and MechanismsStability/Consistency and ReproducibilityAssess ViabilityComparison In Vitro/In Vivo DataTIM-1Advanced Gastric Compartment (TIM-agc)Assess ViabilitySolubility/DialyzabilityAssess ViabilityTransport StudiesAdvantages, Disadvantages and Limitations of the SystemExperimental ReadoutSmall Intestinal Organoid CultureGastric SecretionsRegulation of GLUTag Cell Secretory Activity by NutrientsGeneral ProtocolFeatures and MechanismsExperimental Read OutGeneral ProtocolGastric EmptyingRead Out of the SystemOriginHost-Gut Microbiota InteractionAdvantages, Disadvantages and Limitations of the SystemHistory of the ModelThe Small Intestinal PhaseStability, Consistency and ReproducibilityStatic MethodsDendritic CellsMonitoring ViabilityFeatures and MechanismsIsolating Primary DCs from BloodTroubleshooting Guide for Transport Experiments Across Caco-2 MonolayersStability, Consistency and Reproducibility of the SystemIndirect Positive Isolation of Human CD4+ TThe Use of TIM to Study the Bio-accessibility of NutrientsOperating ProtocolExperimental ReadoutFeatures and MechanismsCritical NotesInfoGest Consensus Method-D Culture SystemsCaco-2/Human Monocyte Derived DCs (Soluble Factors)Advantages, Disadvantages and Limitations of the SystemStability, Consistency and ReproducibilityVerification of Monolayer Integrity by Lucifer Yellow FluxCaco-2/PBMCs (Soluble Factors)Relevance to Human In Vivo SituationOrigin and Some Features of THP-1 and U937 CellsGeneral ProtocolCytokine ProductionDescription of the VTT One Compartment Fermentation ModelGeneral ProtocolsAdvantages and LimitationsPhysical ForcesOriginDescription of TIM-2Medium QualityTrypsinisation and Subculturing of CellsCulture ConditionsAdvantages, Disadvantages and LimitationsHuman Peripheral Blood Mononuclear CellsRelevance to Human In Vivo SituationThe TNO In Vitro Model of the Colon (TIM-2)General ProtocolDynamic Gastric Model (DGM)Advantages, Disadvantages and LimitationsSample PreparationAsses ViabilityThe Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)One Compartment Fermentation ModelDescription of the ModelOriginPreparation of a Food BolusRelevance to Human In Vivo SituationControls to Test Stability and Performance of the ModelGeneral ProtocolQuality in Relation to Other Models with the Same ApplicabilityCell Viability TestingApplications: Advantages and DisadvantagesFeatures and MechanismsRelevance to Human In Vivo SituationThe Ussing ChamberDescription of SHIME®NCI-H716 CellsRelevance to Human In Vivo SituationFreezing CellsControls to Test Viability and Performance of the ModelRelevance to Human In Vivo SituationStability and Reproducibility of the SystemConcept of TIMHistory of the ModelAssess ViabilitySpecial Features of the ModelControls to Test Stability and Performance of the ModelContamination of Cell CulturesControls to Test Stability and Performance of the ModelPBMC-Derived T CellsBiochemistrySmall Intestinal Crypt IsolationGeneral Protocols for Caco-2 CellsValidation of the SystemExperimental ReadoutT Lymphocytes or T-CellsInoculation of Gut Fermentation ModelsPrediction of Glycemic ResponseStudy of Proliferative/Cytotoxic ActivityMonocytes and MacrophagesFunctionality StudiesCo-culture Caco-2 and Dendritic CellsGastric ProcessingReadout of the Ussing Chamber SystemRelevance to Human In Vivo SituationStability and Reproducibility of the SystemAdvantages, Disadvantages and LimitationsOriginRead-Out of the System and How This Information Can Be UsedDC SubsetsRead-Out of the SystemPassaging of Cells in Suspension CultureCaco-2/THP-1 (Soluble Factors)General ProtocolFeatures and MechanismsThe DIDGI® SystemStability/Consistency/ReproducibilityOriginGeneral Advantages, Disadvantages and Limitations of the Ussing Chamber SystemCell Maintenance ProtocolBasic ProtocolSamplesCo-culture Caco-2/Immune CellsMonocyte-Derived DCsPerformance of Secretion and Expression AssaysExperimental ReadoutQuality in Relation to Other Models with the Same ApplicabilityExperimental Protocol for Test CompoundsDynamic Fermentation ModelsGeneral ProtocolProtocol for Polarizing Caco-2 Cells in Tissue Culture InsertsDendritic CellsSpecial FeaturesGeneral Protocol for DGM ExperimentsTemperature ControlCo-stimulationRelevance to Human In Vivo SituationCo-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events)General Cell Culture ProtocolsAnalysis of HGS Biomechanical RelevanceGeneral ProtocolPracticalitiesMaintenance of the GLUTag Cell LineGeneral ProtocolApplications of T Cell CulturesGeneral Advantages and Disadvantages of the ModelIntestinal Rings and Intestinal SegmentsPorcine Ex Vivo Intestinal Segment ModelPharmaceutical-Based ResearchMonitoring ViabilityGastric MotilityMonitoring ViabilityIncubation with Food Components and Digested Food SamplesMeasurement of Transepithelial Electrical Resistance (TEER)Murine GLUTag CellsSTC-1 CellsIsolated and Perfused Intestinal SegmentsTiny TIMAdvantages, Disadvantages and Limitations of the SystemSpecial Features of the ModelExperimental ReadoutsRelevance to Human In Vivo SituationDifferentiation of THP-1 and U937 Monocytes into MacrophagesCo-cultivation of Caco-2 and HT-29MTXAssess ViabilityExperimental Read OutRelevance to the Human In Vivo SituationStability, Consistency and ReproducibilityQuality in Relation to Other Models with the Same ApplicabilityThe Cell Culture HoodCell Culture Terminology (Fig. 1)Serum AdditionSpecial Features/Morphology/ReceptorsSamplingAssess ViabilityOther DC ReadoutsOriginGeneral Protocol for HT29-MTX CellsExperimental Readout of the SystemT Cell Isolation ProtocolsQuality in Relation to Other Models with the Same ApplicabilityGeneral Advantages and Limitations of Intestinal Organoid SystemsGeneral ProtocolRelevance to Human In Vivo SituationStability/Consistency/Reproducibility of the SystemRegulation of GLUTag Cell Secretory Activity by Other SignalsThe Computer-Controlled Multicompartmental Dynamic Model of the Gastrointestinal System SIMGIThe Gastric PhaseOther Models with the Same ApplicabilityStability, Consistency and ReproducibilityRead-Out of the SystemUses of the HGSExperimental Protocol for Test CompoundsAvailability of the SystemGeneral ProtocolValidation of DIDGI® for the Digestion of Infant FormulaStability, Consistency and ReproducibilityIn Vivo Digestion of Infant Formula on PigletsFood-Based ResearchFeatures and MechanismsGeneral ProtocolAdvantages, Disadvantages and LimitationsOriginUssing ChamberGeneral MaintenanceMicroorganisms Survival, Adhesion or InvasionUssing Chamber
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