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The Impact of Food Bioactives on Health

A short course of lectures
«The Impact of Food Bioactives on Health»

Assess Viability History and Special Features of the Model Quality in Relation to Other Models with the Same Applicability Features and Mechanisms Advanced Gastric Compartment (TIM-agc)Stability and Reproducibility of the System Dendritic Cells In Vivo Digestion of Infant Formula on Piglets Sample Preparation Medium Quality General Protocol The TNO Gastro-Intestinal Model (TIM)Description of the Model Origin Solubility/Dialyzability Experimental Read Out Assess Viability Peripheral Blood Mononuclear Cells Use of Digested Food Samples Stability, Consistency and Reproducibility of the System Relevance to Human In Vivo Situation Transport Studies Differentiation of THP-1 and U937 Monocytes into Macrophages Origin Temperature Control Availability of the System Troubleshooting Guide for Transport Experiments Across Caco-2 Monolayers Gastric Secretions Cell Viability Testing -D Culture Systems Differentiation of THP-1 and U937 Monocytes into Dendritic Cells Co-culture of Caco-2/Human Monocyte Derived DCs (Include Contact Dependent Events)Model Description Regulation of GLUTag Cell Secretory Activity by Other Signals Everted Sac Gastrointestinal Organoids Controls: Positive and Negative Caco-2/THP-1 (Soluble Factors)Serum Addition The Simulator of the Human Intestinal Microbial Ecosystem (SHIME®)Controls to Test Stability and Performance of the Model General Protocol for DGM Experiments Biochemistry Isolating Primary DCs from Blood Approaches to Static Digestion Models Experimental Protocol for Test Compounds Special Features The Porcine Ex Vivo Intestinal Segment Model Uses of the DGM Read-Out of the System DC Cell Lines Physical Forces Experimental Readouts STC-1 Cells Read-Out of the System and How This Information Can Be Used General Protocol for HT29-MTX Cells Relevance to Human In Vivo Situation Study of Inflammatory Responses Digestion Conditions Experimental Readout General Protocols T Lymphocytes or T-Cells Readout of the Ussing Chamber System General Protocol of Culturing THP-1 Cells History of the Model Samples Controls to Test Stability and Performance of the Model Maintenance of the GLUTag Cell Line Stability, Consistency and Reproducibility Caco-2/B Cells HT29 Cell Line Freezing Cells Quality in Relation to Other Models with the Same Applicability Read Out of the System Advantages, Disadvantages and Limitations of the System Verification of Monolayer Integrity by Lucifer Yellow Flux Relevance to the Human Situation (L-Cell In Vivo)Assess Viability Microorganisms Survival, Adhesion or Invasion Protein Quality Origins and Design of the DIDGI® System Monitoring Viability Advantages, Disadvantages and Limitations of the System Read-Out of the System and How This Information Can Be Used Description of TIM-2 Asses Viability Stability and Reproducibility of the System General Protocol Availability of the System Experimental Readout Quality in Relation to Other Models with the Same Applicability Monitoring Viability Experimental Protocol for Test Compounds Readout of the Porcine Ex Vivo Intestinal Segment Model Special Features of the Model Stability, Consistency and Reproducibility Relevance to the Human In Vivo Situation Human Peripheral Blood Mononuclear Cells Relevance to Human In Vivo Situation Ussing Chamber Prediction of Glycemic Response The Ussing Chamber Stability/Consistency/Reproducibility of the System Gastric Processing Sample Analysis Stability/Consistency and Reproducibility Origin Static Methods General Protocol Advantages, Disadvantages and Limitations Static Models for Lipid Hydrolysis Stability/Consistency/Reproducibility Origin Critical Notes Experimental Read Out Dendritic Cells CD34+-Derived DCs Experimental Read out Measurement of Transepithelial Electrical Resistance (TEER)General Maintenance Role of Microbiota Advantages and Limitations Origins and Design of the DGM Uses of the HGS Description of the VTT One Compartment Fermentation Model Cell Maintenance Protocol Quality in Relation to Other Models with the Same Applicability Assess Viability Small Intestinal Organoid Passage The Small Intestinal Phase Special Features/Morphology/Receptors Advantages, Disadvantages and Limitations of the System General Protocols for Caco-2 Cells Advantages, Disadvantages and Limitations The Cell Culture Hood General Advantages and Limitations of Intestinal Organoid Systems Controls to Test Stability and Performance of the Model Cell Culture Terminology (Fig. 1)Other Models with the Same Applicability Special Features of the Model Culture Conditions General Protocol Dynamic Fermentation Models In Vitro Studies on Food Bioactives Using DCs (Table 17.3)Food-Based Research TIM-1 PBMC-Derived T Cells Static Models for Protein Hydrolysis General Protocol Dynamic Gastric Model (DGM)The Thawing and Recovery of Cells Functionality Studies Co-stimulation Caco-2 Cell Line General Advantages and Disadvantages of the Model Applications Assess Viability Relevance to Human In Vivo Situation Concept of TIM Cell Maintenance Protocol Basic Protocol Assess Viability The Computer-Controlled Multicompartmental Dynamic Model of the Gastrointestinal System SIMGI History of the Model General Protocol Cells Present in the Intestine Pharmaceutical-Based Research Basic Protocol Origin and Some Features of THP-1 and U937 Cells Critical Notes General Protocol Stability, Consistency and Reproducibility Monocyte-Derived DCs Features and Mechanisms Intestinal Crypt Organoids as Experimental Models Experimental Protocol for Test Compounds Experimental Readout Assess Viability Advantages, Disadvantages and Limitations of the System Porcine Ex Vivo Intestinal Segment Model Gastric Motility Caco-2/Human Monocyte Derived DCs (Soluble Factors)Co-culture Caco-2/Immune Cells Origin Analysis of HGS Biomechanical Relevance Lipids Stability, Consistency and Reproducibility Co-cultivation of Caco-2 and HT-29MTX Tiny TIM Gastric Emptying General Cell Culture Protocols Monitoring Viability The Oral Phase Passaging of Cells in Suspension Culture Trypsinisation and Subculturing of Cells Cell Maintenance Protocol Comparison In Vitro/In Vivo Data Advantages and Disadvantages Validation of the System Origins of the HGS Relevance to Human In Vivo Situation Advantages, Disadvantages and Limitations Validation of DIDGI® for the Digestion of Infant Formula Cytokine Production Advantages, Disadvantages and Limitations The IPEC-J2 Cell Line Origin T Cell Isolation Protocols Monocytes and Macrophages Isolated and Perfused Intestinal Segments Performance of Secretion and Expression Assays Co-culture Caco-2 and Dendritic Cells DC Subsets Other DC Readouts Ussing Chamber General Protocol InfoGest Consensus Method Preparation of a Food Bolus The DIDGI® System Small Intestinal Crypt Isolation General Protocol Small Intestinal Organoid Culture Description of SHIME®Stability, Consistency and Reproducibility Sampling History of the Model Features and Mechanisms The Use of TIM to Study the Bio-accessibility of Nutrients Study of Proliferative/Cytotoxic Activity Murine GLUTag Cells Experimental Readout of the System Practicalities Co-culture Caco-2 and B-cells (Raji)Features and Mechanisms Calculating Transepithelial Resistance Origin, Features and Mechanisms General Protocol NCI-H716 Cells Origin, Features and Mechanisms Origin Incubation with Food Components and Digested Food Samples Protocol for the In Vitro Dynamic Digestion of Infant Formula Using the DIDGI® System Relevance to the Human L-Cell In Vivo Other Static Models The TNO In Vitro Model of the Colon (TIM-2)Features and Mechanisms Relevance to Human In Vivo Situation Intestinal Rings and Intestinal Segments The Gastric Phase pH and CO2 Features and Mechanisms Indirect Positive Isolation of Human CD4+ T General Advantages, Disadvantages and Limitations of the Ussing Chamber System Experimental Readout General Protocol Host-Gut Microbiota Interaction Features and Mechanisms Regulation of GLUTag Cell Secretory Activity by Nutrients Role of ACW Activity on Food Digestion Applications of T Cell Cultures Gastric Compartment Human Gastric Simulator (Riddet Model)Origin Contamination of Cell Cultures Stability and Reproducibility of the System Relevance to Human In Vivo Situation Features and Mechanisms Operating Protocol General Protocol Relevance to Human In Vivo Situation Relevance to Human In Vivo Situation General Protocol General Protocol Applications: Advantages and Disadvantages Caco-2/PBMCs (Soluble Factors)Inoculation of Gut Fermentation Models General Protocol Role of Food Material Properties Relevance to Human In Vivo Situation Controls to Test Viability and Performance of the Model Protocol for Polarizing Caco-2 Cells in Tissue Culture Inserts One Compartment Fermentation Model Relevance to Human In Vivo Situation Read-Out of the System Experimental Readout

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