Advantages, Disadvantages and Limitations

Table of Contents:

The use of cell lines will give good reproducibility of the model system and there are several reports establishing good correlations between this model and human in vivo studies. The two cell lines are easily available and easy to cultivate. The model is quite easy to use and is efficient for screening purposes. The disadvantages of this model is that some of the transporters/carriers found in normal human intestinal epithelium are not expressed by the two cell lines, therefore in the case of transport studies the expression of the necessary molecules should be checked. The ratio between the two cell types will be crucial for the formation of homogeneous mucin layer, which is of great importance for the relevance to the in vivo situation.

Conclusions

The co-culture of Caco-2 and HT-29MTX is a model that is useful for the investigation of transport over intestinal epithelium and for bacterial adhesion and invasion. For such applications the mucin layer and the permeability of the cell layer is crucial and co-culture will give results that are more in compliance with the in vivo situation than monocultures.

References

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