Advantages, Disadvantages and Limitations of the System
The advantage of PBMCs is that it is an easy accessible source of human immune cells, as the cells are isolated form full blood or buffy coats.
Disadvantages and limitations of this model system is the phenotypic differences between peripheral mononuclear cells and immune cells of the intestinal mucosa.
Although PBMCs is an easy accessible source of human immune cells, there are two major points that are important to keep in mind. (1) These cells are blood mononuclear cells that will differ from immune cells found i.e. in intestinal tissues.
(2) When using PBMC in in vitro experiments the cells will lack the environmental stimuli they would have been exposed to under normal in vivo conditions. Both of these points are of great importance for how immune cells responds to different stimuli and should be taken in to account when interpreting the results.
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