Raman spectroscopy is a straightforward technique that has been successfully used to identify microplastic particles in different environmental samples with high reliability (Van Cauwenberghe et al. 2013; Cole et al. 2013; Murray and Cowie 2011; Imhof et al. 2012, 2013). During the analysis with Raman spectroscopy the sample is irradiated with a monochromatic laser source. The laser depends on the system used: available laser wavelengths usually range between 500 and 800 nm. The interaction of the laser light with the molecules and atoms of the sample (vibrational, rotational, and other low-frequency interactions) results in differences in the frequency of the backscattered light when compared to the irradiating laser frequency. This so-called Raman shift can be detected and leads to substance-specific Raman spectra. Since plastic polymers possess characteristic Raman spectra the technique can be applied to identify plastic polymers within minutes by comparison with reference spectra. Raman spectroscopy is a “surface technique”, thus large, visually sorted microplastic particles can be analyzed and the technique can also be coupled with microscopy. Accordingly, micro-Raman spectroscopy allows for the identification of a broad range of size classes down to very small plastic particles of sizes below 1 µm (Cole et al. 2013). If Raman microscopy is combined with Raman spectral imaging it is possible to generate spatial chemical images based on the Raman spectra of a sample. Micro-Raman imaging theoretically allows for the spectral analysis of whole membrane filters at a spatial resolution below 1 µm. This would facilitate the detection of even the smallest microplastic particles in environmental samples, but the applicability for microplastic research has yet to be demonstrated. Raman spectroscopy can also be coupled with confocal laser-scanning microscopy to locate polymer particles within biological tissues with subcellular precision (Cole et al. 2013). One drawback of Raman spectroscopy is that fluorescent samples excited by the laser (e.g. residues of biological origin from samples) cannot be measured as they prevent the generation of interpretable Raman spectra. Generally, lower laser wave lengths, which transfer a high energy result in high signal intensity but also in a high fluorescence. The fluorescence can be minimized by using lasers with higher wave lengths (>1,000 nm). However, the lower energy of the laser results in a lower signal of the polymer sample. More research is necessary to find the optimum laser wave length for a compromise between suppressed fluorescence and low signal intensity for assessments of microplastics in environmental samples. Generally, a purification step of samples to prevent fluorescence is thus recommended prior to measurements for a clear identification of the polymer type of microplastic particles with Raman spectroscopy.