Laying the Foundation for Cytogenetic and Genome Mapping: The Wheat Aneuploid Stocks

Sears (1939, 1954) isolated a range of aneuploid stocks in Chinese Spring wheat that were widely used by the wheat genetics community to dissect and map the polyploid genome of wheat (Fig. 7.2). The monosomic and telosomics were useful for chromosome and arm mapping of genes, and for producing intervarietal substitution lines for the mapping of quantitative traits (Law 1966). Wheat

Fig. 7.2 Molecular cytogenetic mapping of wheat phenotypes and the genome. Steps in targeted physical mapping of genes in wheat. Absence of the marker in the critical nullisomic-tetrasomic, long arm ditelosomic deletion stocks, delS1 and delS2, and presence of the marker in the short arm ditelosomic, deletion stocks delS3 and delS4 maps the gene to the chromosome bin 3, which is defined by the deletion interval spanned by delS3 and delS4 (chromosome segments missing in the aneuploid stocks are shown in hatching) (Originally published in Faris et al. (2002))

nulli-tetrasomics were useful for comparative genetic mapping and determining homoeologous relationships among chromosomes of different genomic origin (Sears 1966). O'Mara (1940) developed an aneuploid approach for mapping alien chromosomes added to wheat.

Gametocidal chromosomes were used to isolate deletion stocks (Endo and Gill 1996) for mapping traits and ESTs (expressed sequence tags) into deletion bins (Qi et al. 2004). This extensive mapping of chromosomes revealed structural and functional differentiation along the centromere and telomere axis and the unequal distribution of genes and recombination towards the distal ends of chromosomes.

The individual telosomic chromosomes, reported by Sears and Sears (1978), have been sequenced (IWGSC 2014) using chromosome genomic methods pioneered by Dolezel and his group (reviewed elsewhere). Nearly 140,000 wheat genes have been identified and mapped to the genome zipper maps of the 21 chromosomes of wheat for genetic mapping and plant breeding applications. Alien chromosomes, present as additions to the wheat genome, also can be isolated and sequenced, providing unlimited markers for alien gene introgression (Tiwari et al. 2014).

Laying the Foundation for Analysis of Chromosome Structure: Chromosome Banding and In Situ Hybridization Methods

By the 1970s, many genes had been mapped on wheat chromosomes and arms in relation to the centromere but little was known about the chromosome structure, especially chromatin differentiation into biological meaningful heterochromatic and euchromatic regions along the chromosome length. In the early 1970s, Gill and Kimber (1974a, b) applied the C-banding technique to wheat and rye revealing remarkable heterochromatic bands that allowed unequivocal identification of individual chromosomes. Combining C-banding with aneuploid stocks, they constructed cytogenetic maps of the chromosome complements of wheat and rye. A nomenclature system was developed to describe the hundreds of chromosome regions into which the wheat genome was partitioned for mapping, and idiograms of the 21 wheat chromosomes were constructed (Gill et al. 1991; Fig. 7.3). The homoeologous chromosomes belonging to A, B, D, and R genomes showed little conservation of chromosome structure, revealing the fast-paced tempo of cereal chromosome evolution in the Triticeae tribe.

Next, newly developed in situ hybridization methods were used to map DNA sequences on chromosomes on a glass slide (Rayburn and Gill 1985). Repetitive DNA mapped to heterochromatic regions was often genome-specific (Rayburn and Gill 1986). Recently, a robust method of mapping single-copy sequences has been applied to wheat (Danilova et al. 2012). A set of 60 full-length cDNA clones for single-copy FISH was developed to rapidly determine the homoeology of any Triticeae chromosome with wheat chromosomes (Danilova et al. 2014; Fig. 7.4).

Fig. 7.3 (Left) The banded karyotype of Chinese Spring wheat. Each chromosome was stained (from left to right) by N-banding, modified C-banding, and C-banding (whole and telosomic chromosomes). Because chromosome 1A, and 3D to 6D do not show any N-bands, they were not identified. (Right) Idiogram of banded chromosomes of Chinese Spring wheat. Band numbers are indicated on the left and FL positions on the right of each chromosome. Hatched bands are not numbered as they were not observed consistently (Originally published in Gill et al. (1991). © Canadian Science Publishing or its licensors)

This set also is very useful for detecting chromosomal rearrangements that may have occurred during Triticeae evolution.

Laying the Foundation for Wheat Chromosome Manipulation: An Integrated Approach for Alien Genetic Transfers

Two basic methods of chromosome manipulation for alien gene introgression have been practiced widely in wheat. Sears (1956) used irradiation to transfer a leaf rust resistance gene from Aegilops umbellulata Zhuk. into wheat. Riley et al. (1968) interfered with the Ph1 gene effect (Riley and Chapman 1958) and used inducedhomoeologous pairing to transfer yellow rust resistance from Ae. comosa Sm. in Sibth. & Sm. into wheat. Later, Sears (1977) produced a mutant at the Ph1 locus and also used a nullisomic 5B chromosome for induced homoeologous pairing and alien gene transfer (Sears 1973).

Fig. 7.4 Idiogram of wheat chromosomes where heterochromatic bands that can be visualized with GAA oligonucleotide probes are in black, pAs1 bands are in green, and cDNA probe positions are shown as red dots. Names of probes that hybridize to more than one homoeologous group are highlighted in red (Originally published in Danilova et al. (2014))

Chromosome banding and in situ hybridization greatly facilitated the characterization of the alien transfers in terms of the identity of the alien and wheat chromosomes involved in the translocations, the breakpoints of wheat–alien translocations, and the amount of the alien chromatin transferred (Friebe et al. 1991; Mukai et al. 1993; for review see Friebe et al. 1996). Qi et al. (2007) presented an integrated chromosome manipulation protocol utilizing modern cytological and molecular methods that has been widely used in many alien transfer experiments.

Looking to the Future

As the wheat genome is sequenced, it will be important to integrate DNA sequences with chromosome features such as heterochromatin and euchromatin, telomeres, and centromeres to gain insight into chromosome structure, function, and evolution, and, especially, how chromatin structure may control gene expression. The Triticeae tribe contains several 100 species, and only a few have been characterized by modern methods. Ideograms should be developed for all Triticeae chromosomes similar to the wheat chromosome idiogram (Fig. 7.3), including the mapping of single-copy

FISH markers for elucidating syntenic relationships (Fig. 7.4). We also need to produce amphiploids, addition, substitution, and compensating Robertsonian translocations involving complete genomes of Triticeae species added to wheat. For accomplishing such a task, the IWGS provides a great platform for coordination of research and sharing of results.

Acknowledgments Contribution no. 15-169-A from the Kansas Agricultural Experiment Station. Research supported by the National Science Foundation and the Wheat Genetics Resource Center Industry/University Cooperative Research Center contract number IIP-1338897.

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