Different Myocardial Tissue Engineering Techniques

The intrinsic capacity of human cardiomyocytes to form spontaneous threedimensional syncytia is a necessary basic principle of myocardial tissue engineering. Combined with engineering techniques, three-dimensional cardiac tissue constructs of the desired geometry and size can be created. Therefore different methods are used, which are described below.

Decellularized Heart Tissue

This technique, first described by Eschenhagen et al. (1997), aims at reconstituting a native heart by decellularizing a whole heart or some parts of it and to reseed it with cardiac cells. First part of this two-step approach is the decellularization process. The decellularization of the native heart can be achieved by Langendorff- perfusion with Triton X-100 and sodium dodecyl substrate (SDS), a procedure, which eradicates almost all cells from the tissue but leaves connective tissue and blood vessels behind. In a second step, the reseeding process, the remaining matrix is then perfused with cardiac cells (Ott et al. 2008). Thereby, the native heart shape can be reconstituted.

< Prev   CONTENTS   Source   Next >