Enzymatic Hydrolysis of Lignin

Lignin-degrading enzymes, such as manganese peroxidase, lignin peroxidase, and laccase, are important components of the fungal deconstruction machinery of ligno- celluloses in nature (Fig. 2.4), but have not been found to improve the hydrolysis of lignocellulosic biomass materials to a significant extent (Martinez et al. 2008). Rather, they can be used to reveal structural features affecting the hydrolysis.

Microorganism

Commercial

preparation

FPase

(FPU/mL)

P-glucosidase

(U/mF)

Protein

(U/mF)

Supplier

Reference

T. longibrachiatwn and A. niger

Celluclast 1.5L

65

12

43

Novozymes

Sprensen et al. (2003) and Garcra-Aparicio et al. (2006)

T. longibrachiatwn and T. reesei

Cellulase 2000L

100

ND

7

Rhodia-Danisco (Vinay. France)

Verardiet al. (2012)

T. longibrachiatwn and T. reesei

Accellerase 1000

93

1632

NA

Novozymes

Fin et al. (2011)

T. longibrachiatwn and T. reesei

Cellulyve 50L

24

ND

34

Fyven (Colombelles, France)

Verardiet al. (2012)

T. longibrachiatwn and T. reesei

Energex L

<5

19

28

Novozymes

Verardiet al. (2012)

T. longibrachiatwn and T. reesei

GC 220

116

215

64

Genencor-Danisco (Rochester. USA)

Verardiet al. (2012)

T. longibrachiatwn and T. reesei

GC 440

<5

70

29

Genencor

Verardiet al. (2012)

T. longibrachiatwn and T. reesei

GC 880

<5

86

43

Genencor

Verardiet al. (2012)

T. longibrachiatwn and T. reesei

Spezyme CP

58.2

128

41

Genencor

Dien et al. (2008)

T. longibrachiatwn and T. reesei

Ultraflo L

<5

20

18

Novozymes

Verardiet al. (2012)

T. longibrachiatwn and T. reesei

Viscozyme L

<5

23

27

Novozymes

Sprensen et al. (2007)

T. longibrachiatwn and T. reesei

Viscostar 150L

33

111

40

Dyadic (Jupiter. USA)

Verardiet al. (2012)

A. niger

Novozym 188

8.5

665

57

Novozymes

Dien et al. (2008)

Lignin biodegradation including enzymatic reactions and oxygen activation (for explanation, see text). Updated from Gutierrez and Martinez

Fig. 2.4 Lignin biodegradation including enzymatic reactions and oxygen activation (for explanation, see text). Updated from Gutierrez and Martinez

 
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