Initial observations showed a nucleus enveloped by typical membranes with pores, condensed chromatin dispersed throughout the nucleoplasm, and a typical nucleolus found in epimastigotes, but not in amastigote or trypomastigote.6,7 The nucleolar material persists throughout the closed mitosis process. It was also shown that the nuclear membrane remains intact throughout mitosis, which is characterized by the appearance of intranuclear microtubules, dispersion of the chromatin, and the appearance of dense plates whose number varies according to the trypanosomatid
Figure 18.3 Freeze-fracture images showing the distribution of the nuclear pores as well as the intramembranous particles seen on the nuclear membrane.
Source: After Esponda P, Souto-Padran T, De Souza W. Fine structure and cytochemistry of the nucleus and the kinetoplast of epimastigotes of Trypanosoma cruzi. J Protozool 1983;30 (1):105—10.
species. The nuclear membrane presents a high density of pores thus indicating a high rate of nucleocytoplasmic interchange (Fig. 18.3).8